Technical Data
H1900-34A
Hepatitis A Virus Cellular Receptor 2 (HAVCR2, Kidney Injury Molecule 3, KIM3, T cell Immunoglobulin and Mucin Domain Containing 3, TIMD3, T cell Immunoglobulin Mucin 3, TIM3)
Description:
CD4 (MIM 186940)-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells and their associated cytokines are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. The 2 types of cells also cross-regulate the functions of the other. TIM3 is a Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice.

Applications:
Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilutions:
Peptide ELISA Titer: 1:32,000
Western Blot: 0.3-1ug/ml ~35kD band observed in Human Tonsil, Thymus, Lymph Node and Spleen lysates (calculated MW of 33.4kD according to NP_116171.3). In transfected HEK293 transiently expressing HAVCR2 a band of ~35kD is observed. This band is not observed in the non-transfected HEK293.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-KWYSHSKEKIQN, from the internal region of human HAVCR2 (NP_116171.3).
Purity:
Purified by peptide affinity chromatography.
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human HAVCR2. Species sequence homology: canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Meyers JH, Sabatos CA, Chakravarti S, Kuchroo VK. The TIM gene family regulates autoimmune and allergic diseases. Trends Mol Med. 2005 Aug;11(8):362-9. Review. PMID: 16002337