Technical Data
H1905-15
Hepatitis B Core Antigen (HBcAg)
Description:
The Hepatitis B virus is one of the most important causative agents of acute and chronic liver disease in humans. HBcAg is a particulate antigen composed of a single polypeptide (p21). It is the replicative form of the virus, and is expressed in the infected cells.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunofluorescence, Immunocytochemistry and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:1000
Immunofluorescence: 1:200
Immunohistochemistry/Immunocytochemistry: Stains infected liver tissues and transfected cultured cells.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k2Q1937Ascites
SizeStorageShippingSourceHost
100ul-20CBlue IceMouse
Concentration:
Not Determined
Immunogen:
Recombinant protein corresponding to HBcAg expressed in E. coli. Epitope: around aa70-80, major C determinant.
Purity:
Ascites
Form
Supplied as a liquid in DMEM, 0.1% sodium azide.
Specificity:
Recognizes HBcAg. Species Crossreactivity: woodchuck hepatitis virus core antigen (WHcAg). Does not recognize duck hepatitis B virus core antigen (DHcAg).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Mol. Immunol. 30: 221-231 (1993). 2. Scand. J. Immunol. 37: 637-643 (1993). 3. Arch. Virol. 134: 393-402 (1994). 4. J. of Medical Virology 53: 127-138 (1997).