Technical Data
Anti-Hepatitis B Virus E Antigen (anti-HBeAg) ELISA Kit, BioAssay™ (Human)
Kits and Assays Storage: 4°CShipping: Blue Ice
The Anti-Hepatitis B Virus E Antigen (HBeAg) ELISA Kit is a qualitative in vitro enzyme immunoassay for the detection of anti-HBe in human serum or plasma.

Principle of the Assay:
The purified Anti-HBe is coated on the solid phase of multi-wells. Serum sample, recombinant HBeAg and horseradish peroxidase labeled with Anti-HBe (conjugated) are added to coated wells, and form a competitive combination. After incubation, if Anti-HBe content is high in the sample, a complex of HBeAg-Anti-HBe-Anti-HBe labeled with HRP will form. The wells are washed to remove the complex, and incubated with substrate (TMB), subsequently producing no color change or little color change. If Anti-HBe is not present in the sample, there will be significant color change. The test is sensitive, reproducible and easy to operate.

Kit Components:
H1910-40J1: Microtiter Plate, 1x96wells
H1910-40J2: Enzyme Conjugate, 1x6ml
H1910-40J3: Positive Control Serum, 1x500ul
H1910-40J4: Negative Control Serum, 1x500ul
H1910-40J5: Wash Buffer (20X), 1x30ml
H1910-40J6: Substrate A, 1x6ml
H1910-40J7: Substrate B, 1x6ml
H1910-40J8: Stop Solution, 1x6ml
H1910-40J9: Neutralization Reagent, 1x6ml

Interpretation of Results:
Cut-off Value (COV) Calculation:

COV = average OD450 of negative controls X 0.5

Positive: OD450 of sample Negative: OD450 of sample COV
Invalid: If the OD of negative control is below or equal to 1.000, the result is invalid. In any event, repeat the test. If the problem persists, contact technical support.

Performance Characteristics:
Sensitivity: 4NCU/ml (per Biological Reference Reagents of Chinese Clinical Test Center), OD 0.600
Specificity: The average OD of 20 normal negative smples is 2.00
Precision: %CV 20% (n=10)

Storage and Stability:
Store all kit components at 4°C. Kit is stable for 6 months.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.