Technical Data
Hepatitis B Core Antigen (HBcAg)
Hepatitis B virus is spherical in shape with a diameter of 42nm. It contains a 27nm partially double stranded DNA core enclosed within a lipoprotein coat. The antigenic activity of the nucleocapsid core is designated as hepatitis B core antigen. The antigens in the outer surface are called as hepatitis B virus surface antigens. Core antigens are localized within the nuclei whereas the surface antigens are present in the cytoplasm of the infected cells. Antibodies to surface antigens appear in circulation at an early stage of infection whereas the antibodies to the core antigens are detected after several weeks.

Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: (Formalin/paraffin): 1:50-1:100 for 30 min at RT. No special pretreatment is required for staining of formalin/paraffin tissues.
Optimal dilution to be determined by researcher.

Positive Control:
Hepatitis B virus infected liver

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
250ul4C (-20C Glycerol)Blue IceHumanRabbit
Not determined
Hepatitis B virus. Cellular Localization: Nuclear
Purified antibody fraction from rabbit anti-serum.
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA and 0.09% sodium azide, before the addition of glycerol to 40%.
Recognizes a protein in the core of hepatitis B virus. Species Crossreactivity: Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Stahl S, et al. (1982) Proc Natl Acad Sci USA, 79:1606.