Technical Data
Hepatocellular Carcinoma, Recombinant, Human (Hemofiltrate Carcinoma Chemokine, HCC-1)
Molecular Biology Storage: -20CShipping: RT
Human Hemofiltrate CC chemokine-1 (HCC-1) was first isolated from the hemofiltrate of patients with chronic renal failure. HCC-1 has a 46% amino acid sequence identity with MIP-1a/MIP-1b and 29-37% homology with other CC chemokines. Recombinant Human HCC-1 is a single, non-glycosylated, polypeptide chain containing 74 amino acids and having a molecular mass of 8411D.

The sequence of the first five N-terminal amino acids was determined and was found to be Thr-Glu-Ser-Ser-Ser.

Dimers and Aggregates:
1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:
HCC-1 is fully biologically active when compared to standard. The Biological activity is calculated by its ability to chemoattract Human monocytes at 5.0-20.0ng/ml.

0.1ng/ug (IEU/ug) of Human HCC-1.

Protein Content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm.
2. Analysis by RP-HPLC, using a calibrated solution of HCC-1 as a Reference Standard.

Reconstitute the lyophilized Human HCC-1 in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Source: E. coli
Purity: 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Concentration: ~0.1mg/ml
Form: Supplied as a lyophilized powder. No additives.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Hemofiltrate CC chemokines with unique biochemical properties: HCC-1/CCL14a and HCC-2/CCL15. Forssmann U, Magert HJ, Escher SE, J Leukoc Biol 2001 Sep;70(3):357-66 2. Posttranslationally processed forms of the human chemokine HCC-1. Richter R, Schulz-Knappe P, Forssmann WG, Biochemistry 2000 Sep 5;39(35):10799-8 05 3. Organization of the chemokine gene cluster on human chromosome 17q11.2 containing the genes for CC chemokine MPIF-1, HCC-2, HCC-1, LEC, and RANTES. Nomiyama H, Fukuda S, Tanase S, J Interferon Cytokine Res 1999 Mar;19(3):227-34 4. Identification of C-C chemokine receptor 1 (CCR1) as the monocyte hemofiltrate C-C chemokine (HCC)-1 receptor. Tsou CL, Gladue RP, Paradis T, Charo IF, J Exp Med 1998 Aug 3;188(3):603-8 5. HCC-1, a novel chemokine from human plasma. Schulz-Knappe P, Magert HJ, Meyer M, Kirchhoff K, J Exp Med 1996 Jan 1;183(1):295-9.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.