Technical Data
H1999-02
Hepatocellular Carcinoma, Recombinant, Human (Hemofiltrate Carcinoma Chemokine, HCC-1)
2ug
10ug
10ug
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Technical Data |
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H1999-02 |
Hepatocellular Carcinoma, Recombinant, Human (Hemofiltrate Carcinoma Chemokine, HCC-1) |
2ug 10ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: RT |
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Human Hemofiltrate CC chemokine-1 (HCC-1) was first isolated from the hemofiltrate of patients with chronic renal failure. HCC-1 has a 46% amino acid sequence identity with MIP-1a/MIP-1b and 29-37% homology with other CC chemokines. Recombinant Human HCC-1 is a single, non-glycosylated, polypeptide chain containing 74 amino acids and having a molecular mass of 8411D. Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Thr-Glu-Ser-Ser-Ser. Dimers and Aggregates: 1% as determined by silver-stained SDS-PAGE gel analysis. Biological Activity: HCC-1 is fully biologically active when compared to standard. The Biological activity is calculated by its ability to chemoattract Human monocytes at 5.0-20.0ng/ml. Endotoxin: 0.1ng/ug (IEU/ug) of Human HCC-1. Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm. 2. Analysis by RP-HPLC, using a calibrated solution of HCC-1 as a Reference Standard. Reconstitution: Reconstitute the lyophilized Human HCC-1 in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. |
Source: E. coli Purity: 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Concentration: ~0.1mg/ml Form: Supplied as a lyophilized powder. No additives. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Hemofiltrate CC chemokines with unique biochemical properties: HCC-1/CCL14a and HCC-2/CCL15. Forssmann U, Magert HJ, Escher SE, J Leukoc Biol 2001 Sep;70(3):357-66 2. Posttranslationally processed forms of the human chemokine HCC-1. Richter R, Schulz-Knappe P, Forssmann WG, Biochemistry 2000 Sep 5;39(35):10799-8 05 3. Organization of the chemokine gene cluster on human chromosome 17q11.2 containing the genes for CC chemokine MPIF-1, HCC-2, HCC-1, LEC, and RANTES. Nomiyama H, Fukuda S, Tanase S, J Interferon Cytokine Res 1999 Mar;19(3):227-34 4. Identification of C-C chemokine receptor 1 (CCR1) as the monocyte hemofiltrate C-C chemokine (HCC)-1 receptor. Tsou CL, Gladue RP, Paradis T, Charo IF, J Exp Med 1998 Aug 3;188(3):603-8 5. HCC-1, a novel chemokine from human plasma. Schulz-Knappe P, Magert HJ, Meyer M, Kirchhoff K, J Exp Med 1996 Jan 1;183(1):295-9.
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