Technical Data
Media
Hepatocyte Basal Medium WAJC 110 (Powder)
H2000
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as mg/liter
Inorganic Salts:
Calcium Chloride•2H2O102.12
Cupric Sulfate•5H2O0.0027467
Ferric Nitrate•9H2O0.834
Magnesium Chloride49.556
Magnesium Sulfate192.608
Potassium Chloride283.1
Potassium Phosphate Mono58.523
Sodium Bicarbonate2.52
Sodium Chloride7592
Sodium Phosphate Dibasic115.1
Zinc Sulfate•7H2O0.14375
Amino Acids:
L-Alanine17.8
L-Asparagine26.42
L-Aspartic Acid26.62
L-Cysteine48.88
L-Glutamic Acid29.42
L-Glutamine292
Glycine15.02
L-Histidine31.04
L-Isoleucine7.872
L-Leucine26.24
L-Lysine58.48
L-Methionine8.95
L-Ornithine50.85
L-Phenylalanine9.91
L-Proline39.06
L-Serine21.02
L-Threonine23.82
L-Tryptophan40.84
L-Tyrosine10.87
L-Valine23.44
Vitamins:
Biotin0.0733
Choline Chloride13.96
Vitamin B121.355
Folic Acid1.324
Myo-Inositol18.02
Nicotinamide0.0366
D-Pantothenate, Ca0.2383
Pyridoxine•HCl0.0616
Riboflavin0.0376
Thiamine•HCl0.337
Other :
AdenineAbsent
Thymidine0.7266
D-Glucose1260
Hypoxanthine4.08
Lipoic Acid0.2063
Phenol Red, Na3.124
Putrescine•2HCl0.322
Sodium Pyruvate220
Total:10.73g/liter
Basal Medium for the growth of primary and serial culture of hepatocytes. The medium substitutes L-Ornithine for L-Arginine, establishing a selective method that favors hepatocyte growth. Supplement with growth factors or fetal calf serum (0.2-2.0% dialyzed).


Directions per Liter: Dissolve 10.73g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, Add sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Off-white to light pink, homogeneous, free flowing powder

Solubility (10.73g/L): Yellow, clear, complete

pH: As Reported

Endotoxin: 10Eu/ml


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Hoshi, H., Kan, M., and Mckeehan, W.L., In Vitro Cell. Dev. Biol. 23:723-732 (1987)