Technical Data
H2005-01
Hepatocyte Growth Factor, Recombinant, Human (HGF, Scatter Factor, SF, Hepatopoietin A)
2ug
10ug
Molecular Biology Storage: -20CShipping: RT
HGF is a mesenchymally derived potent mitogen for mature parenchymal hepatocyte cells and acts as a growth factor for a broad spectrum of tissues and cell types. HGF signals through a transmembrane tyrosine kinase receptor known as MET. Activities of HGF include induction of cell proliferation, motility, morphogenesis, inhibition of cell growth, and enhancement of neuron survival. HGF is a crucial mitogen for liver regeneration processes, especially after partial hepatectomy and other liver injuries. Human and murine HGF are cross-reactive. Human HGF is expressed as a linear 697 amino acid polypeptide precursor glycoprotein. Proteolytic processing of this precursor generates the biologically active form of HGF, which consists of two polypeptide chains (alpha-chain and beta-chain) held by a single disulfide bond resulting in formation of a biologically active heterodimer. The alpha-chain consists of 463 amino acid residues and four kringle domains. The beta-chain consists of 234 amino acid residues.

Biological Activity:
The ED50 was determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells was found to be in the range of 20-40ng/ml.

AA Sequence:
alph achain: QRKRRNTIHE FKKSAKTTLI KIDPALKIKT KKVNTADQCA NRCTRNKGLP FTCKAFVFDK ARKQCLWFPF NSMSSGVKKE FGHEFDLYEN KDYIRNCIIG KGRSYKGTVS ITKSGIKCQP WSSMIPHEHS FLPSSYRGKD LQENYCRNPR GEEGGPWCFT SNPEVRYEVC DIPQCSEVEC MTCNGESYRG LMDHTESGKI CQRWDHQTPH RHKFLPERYP DKGFDDNYCR NPDGQPRPWC YTLDPHTRWE YCAIKTCADN TMNDTDVPLE TTECIQGQGE GYRGTVNTIW NGIPCQRWDS QYPHEHDMTP ENFKCKDLRE NYCRNPDGSE SPWCFTTDPN IRVGYCSQIP NCDMSHGQDC YRGNGKNYMG NLSQTRSGLT CSMWDKNMED LHRHIFWEPD ASKLNENYCR NPDDDAHGPW CYTGNPLIPW DYCPISRCEG DTTPTIVNLD HPVISCAKTK QLR

beta chain: VVNGIPTRTN IGWMVSLRYR NKHICGGSLI KESWVLTARQ CFPSRDLKDY EAWLGIHDVH GRGDEKCKQV LNVSQLVYGP EGSDLVLMKL ARPAVLDDFV STIDLPNYGC TIPEKTSCSV YGWGYTGLIN YDGLLRVAHL YIMGNEKCSQ HHRGKVTLNE SEICAGAEKI GSGPCEGDYG GPLVCEQHKM RMVLGVIVPG RGCAIPNRPG IFVRVAYYAK WIHKIILTYK VPQS

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute by adding sterile ddH2O and a carrier protein. Aliquot and store at -20C. Reconstituted product is stable for 6 months at -20C with a carrier protein. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Country of Origin: USA

Molecular Weight:
80kD
Source: BTI-Tn-5B1-4 High-5 insect cells.
Purity: 98% by SDS-PAGE and HPLC analyses. Endotoxin: 0.1ng/ug (1EU/ug)
Form: Supplied as a lyophilized powder from 0.1M L-arginine HCl, 10mM Tris, 200mM sodium chloride, pH 8.0. Reconstitute with sterile ddH2O and a carrier protein to 0.5mg/ml. Do not vortex

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
US Biological application reference: 1. Ziel., M. et al., (2007) Exp Hematol. 35:1550-1561. 2. Zhang, L. et al., (2011) Anal. Biochem. doi:10.1016/j.ab.2011.01.028. 1. Koch, A.: The SH2-domian-containing inositol 5-phosphatase (SHIP)-2 binds to c-Met directly via tyrosine residue 1356 and involves hepatocyte growth factor (HGF)-induced lamellipodium formation, cell scattering and cell spreading. Oncogene 24(21): 3436-47 (2005). 2.Suzuki, M.: Des-gamma-carboxy prothrombin is a potential autologous growth factor for hepatocellular carcinoma. Journal of Biological Chemistry 280(8): 6409-15 (2/25/2005). 3. Kawamura, K.: N-WASP and WAVE2 acting downstream of phosphatidylinositol 3-kinase are required for myogenic cell migration induced by hepatocyte growth factor. Journal of Biological Chemisstry 279(52): 54862-71 (12/24/2004).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.