		Technical Data
H2005-03	Hepatocyte Growth Factor alpha Chain, Recombinant, Human, Western Blot Control (Hepatocyte Growth Factor, Hepatopoeitin-A, Scatter Factor, SF, HGF, HPTA)	10ug

Growth Factors, Cytokines

Storage: -20°CShipping: Blue Ice

Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase-signalling cascade. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69kD alpha-chain and 34kD beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule.

Source:
Recombinant corresponding to aa32-494 from mature human HGF-A, fused to 6x His-tag at N-terminal, expressed in E. coli.

Applications:
Suitable for use in Western Blot as a control. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Source: E. coli
Purity: Purified (SDS-PAGE)
Concentration: As reported
Form: Supplied as a liquid in 25mM Tris-HCl, pH6.8, 50mM DTT, 1% SDS, 0.1% Bromophenol Blue, 2.5% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Miyazawa, K. et al (1993) Journal of Biological Chemistry 268: 10024-10028. 2. Shimomura T. et al (1992) Cytotechnology 8: 219-229.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.