Technical Data
Hepatocyte Growth Factor, Recombinant, Human (HGF, HGFB, F-TCF, Hepatopoietin A, HPTA, Lung Fibroblast-derived Mitogen, Scatter Factor, SF)
Molecular Biology Storage: -20CShipping: Blue Ice
Hepatocyte Growth Factor Human Recombinant produced in Baculovirus is a heterodimer, non-glycosylated, polypeptide chain consisting an a-chain of 463 amino acids and a-chain of 234 having a total molecular mass of 80kD.

Baculovirus Infected High-5 cells. Amino Acid Sequence agrees with the sequence of native human HGF.

Protein Content:
Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.83 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of HGF as a Reference Standard.

Biological Activity:
The ED50 calculated by the dose-dependent proliferation of monkey 4MBr-5 indicator cells was found to be 20-40ng/ml.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: Baculovirus Infected High-5 cells
Purity: ~95% (RP-HPLC, SDS-PAGE
Concentration: As reported
Form: Supplied as a lyophilized powder from PBS, pH 7.4 0.05% Tween-20. Reconstitute with sterile dH2O to 100ug/ml, which can then be further diluted to other aqueous solutions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.