Technical Data
H2008-51
Hepcidin, 25aa (Hepc, LEAP, Hepatic Bactericidal Protein, Liver Expressed Antimicrobial Peptide)
Description:
Hepcidin (Hepc, hepatic bactericidal protein) or LEAP (liver expressed antimicrobial peptide) is small, cysteine-rich peptide, antimicrobial peptide similar to defensins and thionins. Hepcidin (unprocessed, proprotein in mouse 83aa, rat/human 84aa) are almost exclusively produced in liver. Human hepcidin is produced from 84aa precursor, including a putative 24aa signal peptide. The secreted form of hepcidin in blood and urine consists of C-terminal 20, 22 or 25aa residues. The link between hepcidins and iron metabolism is that hepcidin expression is abolished in mice exhibiting iron-overload due to the targeted disruption of USF2 (upstream stimulatory factor 2) gene resembling the situation in hfe-/- mice. The human gene is located at chromosome 19, in close proximity with Usf2 gene. Hepcidin levels are increased in iron loading and in beta-2 microglobulin knockout mice. Hepcidins are devoid of IRE. Like other antimicrobial peptides, hepcidin is upregulated by lipopolysaccharides (LPS).

Applications:
Suitable for use in ELISA, Immunohistochemistry, Immunofluorescence and Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1-10ug/ml (ECL).
ELISA: 1:100,000. Using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.

Recommended Control Peptide:
H2008-52: Hepcidin, 25aa, Human, Control Peptide (Hepc, LEAP, Hepatic Bactericidal Protein, Liver Expressed Antimicrobial Peptide)

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to13aa within the C-terminus of mature 25aa human HEPC, conjugated to KLH. Species Sequence Homology: mouse: 69%; rat: 61%.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.5, 0.1% BSA.
Specificity:
Recognizes all 3 processed forms of human HEPC. The human sequence is conserved in all 3 human HEPC peptides (20-, 22- and 25-aa C-terminal fragments). May react with the unprocessed larger 60aa pro-HEPC if present in the samples or tissues.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Pigeon, C., et al., JBC 276: 7811-7819 (2001). 2. Park, C.H., et al., JBC 276: 7806-7810 (2001). 3. Krause, A., et al., FEBS Lett. 480: 147-150 (2000). 4. Fleming, R.E., Sly, W.S., PNAS 99: 8160-8162 (2001). 5. Nicolas, G., et al., PNAS 99: 4596-4601 (2002).