Technical Data
Hepcidin, 25aa (Hepc, LEAP, Hepatic Bactericidal Protein, Liver Expressed Antimicrobial Peptide)
Hepcidin (Hepc, hepatic bactericidal protein) or LEAP (liver expressed antimicrobial peptide) is small, cysteine-rich peptide, antimicrobial peptide similar to defensins and thionins. Hepcidin (unprocessed, proprotein in mouse 83aa, rat/human 84aa) are almost exclusively produced in liver. Human hepcidin is produced from 84aa precursor, including a putative 24aa signal peptide. The secreted form of hepcidin in blood and urine consists of C-terminal 20, 22 or 25aa residues. The link between hepcidins and iron metabolism is that hepcidin expression is abolished in mice exhibiting iron-overload due to the targeted disruption of USF2 (upstream stimulatory factor 2) gene resembling the situation in hfe-/- mice. The human gene is located at chromosome 19, in close proximity with Usf2 gene. Hepcidin levels are increased in iron loading and in beta-2 microglobulin knockout mice. Hepcidins are devoid of IRE. Like other antimicrobial peptides, hepcidin is upregulated by lipopolysaccharides (LPS).

Suitable for use in ELISA, Immunohistochemistry, Immunofluorescence and Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1-10ug/ml (ECL).
ELISA: 1:100,000. Using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.

Recommended Control Peptide:
H2008-52: Hepcidin, 25aa, Human, Control Peptide (Hepc, LEAP, Hepatic Bactericidal Protein, Liver Expressed Antimicrobial Peptide)

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
50ug-20°CBlue IceHumanRabbit
Synthetic peptide corresponding to13aa within the C-terminus of mature 25aa human HEPC, conjugated to KLH. Species Sequence Homology: mouse: 69%; rat: 61%.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.5, 0.1% BSA.
Recognizes all 3 processed forms of human HEPC. The human sequence is conserved in all 3 human HEPC peptides (20-, 22- and 25-aa C-terminal fragments). May react with the unprocessed larger 60aa pro-HEPC if present in the samples or tissues
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Pigeon, C., et al., JBC 276: 7811-7819 (2001). 2. Park, C.H., et al., JBC 276: 7806-7810 (2001). 3. Krause, A., et al., FEBS Lett. 480: 147-150 (2000). 4. Fleming, R.E., Sly, W.S., PNAS 99: 8160-8162 (2001). 5. Nicolas, G., et al., PNAS 99: 4596-4601 (2002).