		Technical Data
H2008-54	Hepcidin, CT, 13aa (Hepc, LEAP, Hepatic Bactericidal Protein, Liver Expressed Antimicrobial Peptide)

Description:
Hepcidin (Hepc, hepatic bactericidal protein) or LEAP (liver expressed antimicrobial peptide) is a small, cysteine-rich peptide, antimicrobial peptide similar to defensins and thionins. Hepcidin (unprocessed, proprotein in mouse 83aa, rat/human 84aa) are almost exclusively produced in liver. Human hepcidin is produced from an 84aa precursor, including a putative 24aa signal peptide. The secreted form of hepcidin in blood and urine is consists of C-terminal 20, 22 or 25aa residues. The link between hepcidins and iron metabolism is that hepcidin expression is abolished in mice exhibiting iron-overload due to the targeted disruption of USF2 (upstream stimulatory factor 2) gene resembling the situation in hfe-/- mice. The human gene is located at chromosome 19, in close proximity with Usf2 gene. Hepcidin levels are increased in iron loading and in beta-2 microglobulin knockout mice. Hepcidins are devoid of IRE. Like other antimicrobial peptides, hepcidin is upregulated by lipopolysaccharides (LPS).

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1-10ug/ml (ECL).
ELISA: 1:100,000. Using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.

Recommended Control Peptide:
H2008-55: Hepcidin, Mouse, 13aa, CT, Control Peptide

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Mouse	Rabbit

Concentration:

~1mg/ml

Immunogen:

Synthetic peptide corresponding to13aa within the C-terminus of mature mouse HEPC. Species Sequence Homology: rat, 92%; human, 69% (20-, 22- and 25-aa C-terminal fragments).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, 0.1% BSA.

Specificity:

Recognizes mouse Hepcidin. May react with the unprocessed larger 60aa pro-HEPC.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Peyssonnaux, C., Blood 107: 3727-3732 (2006) - IF. 2. Peyssonnaux, C., J. Clin. Invest. 117: 1926-1932 (2007) - IHC. 3. Duvigneau, J.C., et al., Lab. Invest. 88: 70-77 (2007). 4. Dallalio, G., 2003, British J. Haematol. 122: 996-1000 (2003), WB. 3. Peyssonnaux, C., J. Clin. Invest. 117: 1926 (2007), IHC. 5. Sow, F.B., J. Leukoc. Biol. 82: 934-945 (2007), WB. 6. Peyssonnaux, C., Blood, 107: 3727-3732 (2006) WB, IF. 7. Huang, Y-S., 2006, Pediatr. Res. May 2006; 59: 662-666 (2006), IHC. 8. Pigeon, C., et al., JBC 276: 7811-7819 (2001). 9. Park, C.H., et al., JBC 276: 7806-7810 (2001). 10. Krause, A., et al., FEBS Lett. 480: 147-150 (2000). 11. Fleming, R.E. and Sly, W.S., PNAS 99: 8160-8162 (2001). 12. Nicolas, G., et al., PNAS 99: 4596-4601 (2002).