Technical Data
HEPES Free Acid
Biochemicals Storage: RTShipping: RT
HEPES is a biological buffer (zwitterionic) designed by Good, et al., and typically referred to as Good’s buffer useful in cell culture media formulations. Selection of biological buffer systems should include the following criteria: exclusion by biological membranes, low absorption between 240 and 700nm, chemically stable, and stable to temperature and concentration changes.

Appearance: White, crystalline powder

Purity: 99.0%

Identity (IR): Conform to reference

Solubility: Colorless, clear, complete

A260nm (1M): 0.05

A280nm (1M): 0.05

A405nm (1M): 0.05

pH: 5.2±0.5

pKa: 7.55 ± 0.2

Water (KF): 0.5%

Residue on Ignition: 0.5%

Heavy Metals: 0.001%

RNase, DNase, Protease: None Detected

Endotoxin: 1EU/ml

Certificate of Origin: China

CAS Number:

Molecular Formula:

Molecular Weight:
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Good, N. E., et al., Biochemistry 5:467 (1966)

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.