Technical Data
H2034-86
Hhex, CT (Hematopoietically-expressed Homeobox Protein Hhex, Homeobox Protein Hex hex)
Description:
Hematopoietically expressed homeobox (hhex) is so named for its homeobox DNA-binding domain, capable of recognizing the DNA sequence 5'-ATTAA-3', as determined by similarity through the Uniprot database. Hhex works in parallel with the transcription factor gene scl to regulate early endothelial and blood differentiation in zebrafish. While premature and ectopic expression of early endothelial and blood differentiation genes can be shown via the ectopic expression of hhex by injection of a DNA construct, hhex deficiency studies have shown that hhex is not essential to endothelial and blood differentiation. Scl has been shown to compensate for the missing function of hhex, and hhex and scl have been demonstrated to cross-regulate each other's expression. Hhex is also suggested to regulate early embryonic patterning. Hhex is known to regulate liver development and digestive organ chirality in zebrafish.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:5000-1:20,000
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Control Peptide: H2034-86P

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
250ul-20CBlue IceZebrafishRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to the C-terminal region of zebrafish Hhex.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 40mM MOPS, pH 7.5, 0.1% BSA, 0.05% sodium azide, 50% glycerol.
Specificity:
Recognizes zebrafish Hhex.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Liao, W et al (2000) Development 127:4303-4313. 2. Ho, C-Y et al (1999) Curr. Biol. 9:1131-1134. 3. Wallace, Kn et al (2001) Genesis 30:141-143.