Technical Data
H5080-06
Histamine
Description:
Histamine is a neurotransmitter in the central nervous system a well as a mast cell constituent. In addition, histamine is produced by endocrine cells (ECL-cells) in the oxynthic mucosa of the stomach.

Applications:
Suitable for use in Immunohistochemistry and Immunocytochemistry. Not suitable for use on samples fixed with aldehydes. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:1000-1:4000 on brain and stomach using PAP or ABC. Suggested fixative is 4% EDAC or EDAC + PFA.
Immunocytochemistry: 1:200-1:5000 (cultured cells 2H3 mastocytoma)
Note: Preabsorption of the antiserum with a histamine-BSA or histamine-ovalbumin conjugate (1-100ug/ml) completely abolished non-specific binding.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20°CBlue IceRabbit
Concentration:
Not determined
Immunogen:
Histamine conjugated to KLH with EDAC.
Purity:
Serum.
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide.
Specificity:
Recognizes Histamine. No reactivity to L-histadine containing peptides. Species Crossreactivity: Vertebrates and invertebrates (human, rat, mouse, frog, fish, mollusks, and flatworm).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Panula et al. PNAS (1984) 81:2572-2576. 2. Hakanson et al. Histochemistry (1986) 86:5-17. 3. Panula et al. J. Histoch Cytoch (1988) 36:259-269. 4. Panula, et al. Neuroscience (1990) 34:127-132.