Technical Data
H5099-13
Histidine Tag
Description:
Applications:
Suitable for use in Immunohistochemistry, ELISA, Immunoprecipitation and Western Blot Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: Frozen sections
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-65W: IgG, F(ab)2 (HRP) Pab Rb xMo
I1904-66C: IgG, F(ab)2X-adsorbed (HRP) Pab Rb xMo

Hybridoma:
Spleen cells from immunized Balb/c mice were fused with cells of the mouse NS1 myeloma cell line.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG19A172Affinity Purified
SizeStorageShippingSourceHost
1mg4C (-20C Glycerol)Blue IceMouse
Concentration:
~1mg/ml
Immunogen:
PAX6 transcription factor linked to histidine tag
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH7.4, 0.09% sodium azide.
Specificity:
Recognizes Histidine tag proteins containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Tracks the purification of proteins expressed in bacteria, yeast or insect cells. It reacts against all His tagged proteins. In Western Blot of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Conrath, K. et al. (2001) Camel single-domain antibodies as modular building units in bispecific and bivalent antibody constructs. J. Biol.Chem. 276 (10): 7346 - 7350. 2. Bahi, A. et al. (2004) CD81-induced behavioural changes during chronic cocaine administration: in vivo gene delivery with regulatable lentivirus. Eur. J. Neurosci. 19:1621-1633.