Technical Data
H5099-16A
His-Tag (6-Histidine) (Biotin)
Description:
Insertion of a 6-histidine tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the His-tagged product. Such proteins are purified by immobilized metal ion afftinity chromatography which makes use of its high affinity for transition metal ion.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 1:500-1:1000; Tested using histidine tagged ubiquitin.
Western Blot: 1:1000; Tested using His-tagged recombinant protein control. H5099-16A has been shown not to crossreact with any endogenous products when using bacterial extracts. Some crossreactivity may be seen with extracts of insect or mammalian cells.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Reagent:
S7973-97B: Streptavidin (HRP)

Hybridoma:
NS1 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG13H2201Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
PAX6 transcription factor linked to histidine tag.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Labeled with Biotin.
Specificity:
Recognizes proteins containing the motif H-H-H-H-H-H and proteins containing histidine tags. Reacts against all His tagged proteins so far tested. May be used to track the purification of proteins expressed in bacteria, yeast or insect cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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