Technical Data
H5099-16B
His-Tag (6-Histidine) (HRP)
Description:
Insertion of a 6-histidine tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the His-tagged product. Such proteins are purified by immobilized metal ion afftinity chromatography which makes use of its high affinity for transition metal ion.

Applications:
Suitable for use in Western Blot and ELISA. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000-1:5000
ELISA: 1:20-1:1000
Optimal working dilutions to be determined by researcher.

Hybridoma:
NS1 myeloma cells with spleen cells from Balb/c mice.

Source:
Tissue cuture supernatant

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG13H2201Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
PAX6 transcription factor linked to histidine tag.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.01% Thiomersal, HRP stabilizer. Labeled with horseradish peroxidase (HRP).
Specificity:
Recognizes proteins containing the motif H-H-H-H-H-H and proteins containing histidine tags. Recognizes all His tagged proteins so far tested. May be used to track the purification of proteins expressed in bacteria, yeast or insect cells. In Western Blot of bacterial extracts, does not crossreact with any endogenous products. Some crossreactivity may be seen with extracts of insect or mammalian cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Conrath, K. et al. (2001) J. Biol. Chem. 276: 7346-7350. 2. Suen, J.L. et al. (2001) Immunol. 103: 301-309. 3. Hoffmann, S.C. et al. (2007) J. Biol. Chem. 282: 22370-22375. 4. Zheng, J. et al. (2007) BMC Immunol. 8: 4. 5. Bahi, A. & Dreyer, J.L. (2008) Genes Brain Behav. 7: 244-256. 6. Wrighton, K.H. et al. (2009) J. Biol. Chem. 284: 9755-9763.