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Technical Data |
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H5100-02 |
Histidine |
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Description: Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications have not been tested. Recommended Dilution: Immunohistochemistry: 1:1000-1:2500 Optimal dilutions to be determined by the researcher. Compound: Crossreactivity Ratio: Histidine-G-BSA 1 Carnosine-G-BSA 1: > 100,000 Histamine-G-BSA 1: > 100,000 G: Glutaraldehyde BSA: Bovine Serum Albumin The crossreactivities were determined using ELISA, at concentration/unconjugated or conjugated amino acid concentration at half displacement. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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