Technical Data
H5109-47K
HDAC2 (HDAC-2, Histone Deacetylase 2, RPD3, YAF1)
Description:
HDAC2 is a class I mammalian histone deacetylase containing 488 amino acid residues with an apparent size of ~55kD by denaturing SDS-PAGE. HDAC2 has been shown to interact directly with transcription repressor complexes and the nuclear receptor corepressor N-CoR.

Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 0.5-4ug/ml
Immunocytochemistry:10-20ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.2mg/ml
Immunogen:
Synthetic peptide surrounding amino acid 479 of human HDAC2.
Purity:
Purified by Immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.5% BSA, 0.01% thimerosal, 30% glycerol.
Specificity:
Recognizes human HDAC2 at 55kD. Species crossreactivity: mouse, rat, chicken, hamster, canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.