Technical Data
HDAC3, NT (Histone Deacetylase 3, HD3, RPD3, RPD3-2, SMAP45)
Histone deacetylase 3 belongs to the histone deacetylase family type 1 which actively deacetylate Histones. Currently 3 known isoforms exists (HDAC3, HDAC3A and HDAC3C) (1). HDAC3 represses transcription and binds transcription factor YY1 to form a large complex. N-Cor binds HDAC3 in a different manner than other HDACs. In addition to N-Cor, HDAC3 will form a complex with corepressor SMRT (2). HDAC3 is also known to interact with DAXX, HDAC10 and DACH1.

Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-2000
Immunohistochemistry: 1:100-1:250
Immunocytochemistry: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
100ul-20°CBlue IceHumanRabbit
Not determined
Synthetic peptide corresponding to residues near the N-terminus of Histone deacetylase 3.
Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
Recognizes human Histone Deacetylase 3. Species Crossreactivity: Mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wen-Ming Yang, et al. 272, (44), 28001-28007, 1997 2. Li, J., et al, EMBO J. 19, pp. 4342