Technical Data
H5110
Histone H1
Description:
Autoantibodies to histone antigens have been described in patients with idiopathic and drug-induced SLE, rheumatoid arthritis, and other conditions. The presence of autoantibodies to histones are frequently found in several rheumatic disorders. In one study, the predominant responses to histones in SLE sera were to H1, H2b, and H3. Marked elevations of binding occurred to H1 and H2b in 33% of patients, while 25% showed higher binding to H3. The same study showed the highest anti-histone reactivity to be in patients with rheumatoid arthritis with vasculitis, while the highest reactivity in SLE sera was in those patients with a history of photosensitivity.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunoflourescence. Other applications not tested.

Recommended Dilution:
Immunoprecipitation: 1:3000-1:15,000
Optimal dilutions to be determined by the researcher.

Molecular Weights of Histones:
Lysine Rich (H1, f1): ~ 21,500
Slightly Lysine Rich (H2a, f2a2): 14,004
Slightly Lysine Rich (H2b, f2b): 13,774
Arginine Rich (H3, f3): 15,324
Arginine Rich (H4, f2a1): 11,282

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
250ug-20CBlue IceBovineSheep
Concentration:
~1mg/ml
Immunogen:
Histone 1, calf whole protein complexed to ribosomal RNA.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added.
Specificity:
Recognizes bovine Histone-H-1: 100%. No reaction with Histone-H-2b , Histone-H-3 or Histone-H-4. Very low crossreactivity with Histone-H-core.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.