Technical Data
H5110-03D
H1 histone family, member 2, phosphorylated (Ser27) (H1.B, histone cluster 1, H1c, H1.2, H1F2, Histone H1.2, Histone H1d, MGC3992)
Description:
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into 30nm chromatin fibers and higher order structures.

Applications:
Suitable for use in Western Blot and Luminex. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1ug/ml. Detected H5110-03D in untreated and cocemid-treated HeLa acid extract.
Optimal dilutions to be determined by the researcher.

Positive Control:
Untreated and colcemid-treated HeLa acid extract.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic linear peptide corresponding to the region at and around phosphorylated serine 27 of histone H1.B (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Specificity:
Recognizes phosphorylated serine 27 at and around the N-terminal region of human histone H1.B at ~35kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.