Technical Data
H5110-08B2
H2AFX, CT (Histone H2A.x, H2a/x, H2AX)
Description:
H2AFX is basic nuclear protein that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
~0.25mg/ml
Immunogen:
KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa114-143 from the C-terminal region of human H2AFX.
Purity:
Purified by Protein A and peptide affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human H2AFX.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Yamagata,K.et.al., Biochem. Biophys. Res. Commun. 390(4), 1355-1360 (2009).