Technical Data
H5110-10
Histone, Core
Description:
Autoantibodies to histone antigens have been described in patients with idiopathic and drug-induced SLE, rheumatoid arthritis and other conditions. The presence of autoantibodies to histones are frequently found in several rheumatic disorders. In one study, the predominant responses to histones in SLE sera were to H1, H2b, and H3. Marked elevations of binding occurred to H1 and H2b in 33% of patients, while 25% showed higher binding to H3. The same study showed the highest anti-histone reactivity to be in patients with rheumatoid arthritis with vasculitis, while the highest reactivity in SLE sera was in those patients with a history of photosensitivity. In diploid eukaryotic cells, the chromatin fibers are about 20nm in diameter. They consist of two major components in equal amounts, DNA and basic proteins called histones. Histones are believed to be regularly arranged in the deep grove of the DNA helix. The recurring positive charges of the histones form electrostatic associations with the negatively charged phosphate groups of DNA making the DNA more stable and flexible. This allows for the supercoiling of the chromatin fibers.

Applications:
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applilcations have not been tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Molecular Weights of Histones:
Lysine Rich (H1, f1): ~21,500
Slightly Lysine Rich (H2a, f2a2): 14,004
Slightly Lysine Rich (H2b, f2b): 13,774
Arginine Rich (H3, f3): 15,324
Arginine Rich (H4, f2a1): 11,282

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
250ug-20°CBlue IceBovineSheep
Concentration:
~1mg/ml
Immunogen:
Intact calf Histone core complex to rRNA
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4. No preservatives added.
Specificity:
Recognizes all bovine histones at ~15kD. Species Crossreactivity: human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application references: 1.Poitras, M.F. et al., (2007) Neuroscience 148:198–211 (WB). 2. Yuan, M. et al., (2009) Experimental Neurology 217:210-218. 3. Li, X. et al., (2010) J Neurochem 113:1012-1022. 1. Bradbury, E.M., Histone Nomenclature in the Structure and Function of Chromatin, (Fitzsimmins, D.W. and Walstenholme, G.E.W., eds., American Elsevier, New York ) CIBA Foundation Symposium 28: 4 (1975). 2. Elgin, S.C.R. and Weinbrauh, H.: “Chromosomal Proteins and Chromatin Structure”, Annual Review of Biochemistry, p.725, Annual Reviews, Palo Alto p.725 (1975). 3. Johns, E.W. and Butler, J.A.V., “Further Fractionations of Histones from Calf Thymus”, Biochem. J. 82: 15 (1962).