Technical Data
H5110-12M5
Histone H3 (Histone H3.3, H3 Histone Family 3A, H3F3A, H3F3B) (HRP)
Description:
Modulation of chromatin structure plays an important role in the regulation of transcription in eukary-otes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3 and H4), is the primary building block of chromatin (1). The amino-
terminal tails of core histones undergo various post-transla-tional modifications, including acetylation, phosphorylation, methylation and ubiquitination (2-5). These modifications
occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15 and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18 and 23. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28 and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation of Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mam-
malian cells reveals mitotic phosphorylation of H3 Thr3 in prophase and its dephosphorylation during anaphase (11).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG10E27Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to the carboxy-terminal residues of human histone H3 (KLH).
Purity:
Purified
Form
Supplied as liquid in PBS, pH 7.4, 2mg/ml BSA, 50% glycerol, 50% glycerol. Labeled with HRP.
Specificity:
Recongnizes endogenous levels of human total histone H3 protein. Species crossreactivity: mouse, rat, monkey, pig, hamster, bovine, zebrafish.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79, 2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41, 3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5, 4. Cheung, P. et al. (2000) Cell 103, 263-71, 5. Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73, 6. Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9, 7. Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13, 8. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60, 9. Goto, H. et al. (1999) J Biol Chem 274, 25543-9, 10. Preuss, U. et al. (2003) Nucleic Acids Res 31, 878-85, 11. Dai, J. et al. (2005) Genes Dev 19, 472-88.