Technical Data
H5110-13A
Histone H3, acetylated (Lys9)
Description:
Applications:
Suitable for use in Western Blot, Chromatin IP and Beadlyte Histone-Peptide Specificity Assay. Other applications not tested.

Recommended Dilution:
Immunoblot Analysis: A 1:5000-1:10,000 dilution of this lot detected acetyl-Histone H3 (Lys9) in acid extracts from sodium butyrate treated HeLa cells.
Chromatin Immunoprecipitation (ChIP): An independent laboratory has shown this antibody preferentially immunoprecipitates chromatin from wild type yeast and not from a yeast strain containing a Lysine substitution to Alanine at residue 9.
Beadlyte Histone-Peptide Specificity Assay: A 1:1000-1:5000 dilution of this lot was incubated with histone H3 peptides containing various modifications conjugated to Luminex® microspheres. No crossreactivity with peptides containing acetyl-lysine 14 or acetyl-lysine 27 was observed.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20°CBlue IceRabbit
Concentration:
Not determined
Immunogen:
Ovalbumin-conjugated, synthetic peptide (KQTARAcKSTGGK-C) corresponding to aa4-14 of yeast histone H3 acetylated on lysine 9, with a C-terminal cysteine added for conjugation purposes.
Purity:
Serum
Form
Supplied as a liquid containing 0.05% sodium azide before the addition of glycerol to 30%.
Specificity:
Recognizes acetyl-histone H3 (Lys9), Mr 17kD. An additional unknown protein was detected at Mr 23kD. Species Crossreactivity: Yeast and human. Broad species crossreactivity is expected.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Suka, N., et al., Mol. Cell 8: 473, 2001