Technical Data
H5110-13K
Histone H3, phosphorylated (Ser10)
Description:
Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranslational modifications including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20. Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation
of Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostain- ing with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation of H3 Thr3 in prophase and its dephosphorylation during anaphase.

Applications:
Suitable for use in ELISA, Western Blot, Immunofluorescence, and Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Immunofluorescence (IF-IC): 1:100
Immunofluorescence (IF-F): 1:100
Flow Cytometry: 1:25
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG13A50Ascites
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
Not Determined
Immunogen:
Synthetic phosphopeptide corresponding to residues around Ser10 of human histone H3.
Purity:
Ascites
Form
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Specificity:
Recognizes endogenous levels of human histone H3 only when phosphorylated at Ser10 at 17kD. Does not crossreact with other phosphorylatied histones or acetylated of histone H3. Species Crossreactivity: mouse and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Workman, J.L. and Kingston, R.E. (1998) Annu. Rev. Biochem. 67, 545-579. 2. Hansen, J.C. et al. (1998) Biochemistry 37, 17637-17641. 3. Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-45. 4. Cheung, P. et al. (2000) Cell 103, 263-271. 5. Thorne, A.W. et al. (1990) Eur. J. Biochem. 193, 701-713. 6. Hendzel, M.J. et al. (1997) Chromosoma 106, 348-360.