Technical Data
H5110-14A
Histone H3, acetylated (Lys9)
Description:
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs). Acetylation of lysine14 is commonly seen in genes that are being actively transcribed into RNA.

Applications:
Suitable for use in Western and Dot Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:500. Detects acetylated histone H3 on 10ug of HEK293 lysates.
Dot Blot: 0.5ug/ml. Detects 50ng of mono-acetylated peptide corresponding to position Lys9 in the N-terminal sequence of Histone H3. Does not detect the mono-acetylated peptide corresponding to acetyl-lysine at position 14 or unacetylated Histone H3.
Optimal dilutions to be determined by the researcher.

Positive Control:
TSA-treated HeLa and NIH/3T3 cells.

Recommended Secondary Antibodies:
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb
I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to aa1-12, ARTKQTAR(AcK)STG-C, where (AcK) denotes acetyl-lysine of human Histone H3, conjugated to KLH (NP_003484). Peptide sequence is identical in a wide range of animal and plant species.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.
Specificity:
Recognizes human Histone H3 (Mr 17kD) acetylated at position Lys 9. Species Crossreactivity: mouse, rat and Eukaryote.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Su, R.C., et al., Nat. Genet. 36: 502-506 (2004). 2. Li, J., et al., J. Biol. Chem. 278: 1183-1191 (2003). 3. Bjerling, P., et al., Mol. Cell Biol. 22: 2170-2181 (2002). 4. Kruhlak, M., et al., J. Biol. Chem. 276: 38,307-38,319 (2001). 5. Nowak, S. & Corces, V., Genes Dev. 14: 3003-3013 (2000). 6. Salvador, L.M., et al., J. Biol. Chem. 276: 40,146-40,155 (2001). 7. Zhong, S., et al., J. Biol. Chem. 276: 33,213-33,219 (2001).