Technical Data
Histone H3, trimethyl (Lys27)
The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation and ubiquitination (1).

Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence (IC): 1:400
Western Blot: 1:1000
Immunoprecipitation: 1:50
Chromatin Immunoprecipitation: 1:25
Immunohistochemistry (paraffin): 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ul-20CBlue IceRabbit
Not determined
Synthetic peptide corresponding to the amino terminus of histone H3 in which Lys27 is tri-methylated (KLH). Species Sequence Homology: Xenopus (100%).
Purified by Immunoaffinity chromatography.
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Recognizes endogenous levels of human histone H3 only when tri-methylated on Lys27. Does not crossreact with non-methylated, mono-methylated or di-methylated Lys27. In addition, the antibody does not crossreact with mono-methylated, di-methylated or tri-methylated histone H3 at Lys4, Lys9, Lys36 or Histone H4 at Lys20. Species Crossreactivity: human, mouse, rat and monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Peterson, C.L. & Laniel, M.A., Curr. Biol. 14: R546z-R551 (2004). 2. Kubicek, S., et al., Ernst Schering Res. Found Work-shop, 1-27 (2006). 3. Lin, W. & Dent, S.Y., Curr. Opin. Genet. Dev. 16: 137-142 (2006). 4. Lee, D.Y., et al., Endocr. Rev. 26: 147-170 (2005). 5. Daniel, J.A., et al., Cell Cycle 4: 919-926 (2005). 6. Shi, X., et al., Nature 442: 96-99 (2006). 7. Wysocka, J., et al., Nature 442: 86-90 (2006). 8. Wysocka, J., et al., Cell 121: 859-872 (2005). 9. Trojer, P. & Reinberg, D., Cell 125: 213-217 (2006).