Technical Data
H5110-17D
Histone H4, Dimethyl (Lys20)
Description:
Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 0.01-0.1ug/ml detects methyl-histone H4 in HeLa acid extracts. Minimal crossreactivity with unmethylated recombinant Histone H4 is observed if used at these concentrations.
Immunohistochemistry: Stains Drosophila polytene chromosomes as reported by an independent laboratory.
Immunoprecipitation: Immunoprecipitates methly-Histone H4 from HeLa cell lysates as reported by an independent laboratory.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG13J7Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to aa17-28 (RHR[di-meK]VLRDNIQGC) of human histone H4, conjugated to KLH
Purity:
Purified
Form
Supplied as a liquid in PBS.
Specificity:
Recognizes human methyl-Histone H4, Mr 10kD. Species Crossreactivity: mouse, rat, and bovine. Broad species crossreactivity is expected based on sequence homology. Does not crossreact with yeast or Tetrahymena.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Nishioka, K., et al., Mol. Cell 9: 12011213 (2002).