Technical Data
H5110-18
Histone H4
Description:
Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranslational modifications including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20. Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000; incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Immunoprecipitation: 1:50
Immunohistochemistry (Paraffin): 1:25
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
As reported.
Immunogen:
Synthetic peptide corresponding to the amino-terminal sequence of human histone H4 (11kD) (KLH).
Purity:
Purified by Protein A and immunoaffinity chromatography.
Form
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA and 50% glycerol. No preservative added.
Specificity:
Recognizes endogenous levels of the human histone H4 protein. Does not crossreact with other histones. Species crossreactivity: mouse, rat, monkey, S. cerevisiae, Drosophila and Zebrafish.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Workman, J.L. and Kingston, R.E. (1998) Annu. Rev. Biochem. 67: 545-579. 2. Hansen, J.C., et al. (1998) Biochemistry 37: 17,637-17,641. 3. Strahl, B.D. and Allis, C.D. (2000) Nature 403: 41-45. 4. Cheung, P., et al. (2000) Cell 103: 263-271. 5. Thorne, A.W., et al. (1990) Eur. J. Biochem. 193: 701-713. 6. Hendzel, M.J., et al. (1997) Chromosoma 106: 348-360.