Technical Data
H5305
Histones, pan
Description:
There are 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail histones are involved with the structure of the nucleosomes of the 'beads on a string' structure.

Applications:
Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: 1-5ug/ml
Western Blot: Detects a band at ~20kD
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG12Q2283Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Purified nuclear fractions of HeLa cells
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide
Specificity:
Recognizes human histones H1, H2A, H2B, H3 and H4 with comparable sensitivity. Binds specifically to an antigen determinant, which is present on all five histone proteins. Species Crossreactivity: bovine, rat, Xenopus, opossum and hamster.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kutney, S., et al. (2004). J. Biol. Chem. 279(29): 30850-30855.