Technical Data
H6001-10C
HIV-1 (Human Immunodeficiency Virus-1) (FITC)
Description:
HIV is a member of the genus Lentivirus, part of the family of Retroviridae, which use reverse transcriptase to convert the viral RNA genome into double stranded DNA. This is then integrated into the cells DNA, which is followed by latency or active HIV replication. HIV primarily infects CD4+ T lymphocytes, resulting in their dramatic decline. When CD4+ T cell numbers decline below a critical level, cell-mediated immunity is lost, and the body becomes progressively more susceptible to opportunistic infections. If untreated, this can lead to AIDS (Acquired Immunodeficiency Syndrome).

Applications:
Suitable for use in Immunofluorescence. Other applications not tested.

Recommended Dilutions:
Direct FA: 1:10-1:50. Acetone fixation of the antigen source is recommended prior to staining.
Optimal dilutions to be determined by the researcher.

Titer:
Indirect Immunofluorescence: 1:1000

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
1ml-20CBlue IceGoat
Concentration:
~4-5mg/ml
Immunogen:
Human isolate, confirmed.
Purity:
Purified IgG fraction to a purity of > 95%.
Form
Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.1% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Specificity:
Purified virions, disrupted. Uninfected Cell Reactivity: Does not react with T4+ human lymphocytes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.