Technical Data
H6003-34G2
HIV-1 gp120 (Biotin) (Human Immunodeficiency Virus Type 1)
Description:
Human immunodeficiency virus type 1 (HIV-1) entry into target cells is directed by the envelope (Env) glycoproteins, which are present on the surface of HIV-1 virion or infected cells in the form of trimers consisting of gp120/gp41 complexes. The surface subunit, gp120, initiates the entry process by interacting sequentially with the CD4 receptor and a co-receptor CCR5 or CXCR4, thereby inducing a conformational change that allows the transmembrane (TM) gp41 subunit to mediate fusion between viral and target cell membranes. Cleavage of Env into its gp120 and gp41 components is necessary for activation of its fusogenic activity.

Applications:
Suitable for use in ELISA, Western Blot, Immunocytochemistry and FACS. Other applications not tested.

Recommended Dilution:
ELISA: 1:5000
Western Blot: 0.1-0.5ug/ml
Optimal dilutions to be determined by the researcher.

Biotin/Protein ratio: 10-20

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG5i24Highly Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
HIV-1 gp120 (Human Immunodeficiency Virus Type 1)
Purity:
Purified by gel exclusion chromatography. ( 95% pure).
Form
Supplied as a liquid in PBS, 2.5% BSA, 0.02% sodium azide. Labeled with Biotin.
Specificity:
Recognizes native and recombinant HIV-1 gp120.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.