		Technical Data
H6003-34G3	HIV-1 gp120 (Human Immunodeficiency Virus Type 1) (FITC)

Description:
Applications:
Suitable for use in Direct Immunofluorescence and Dot Blot. Other applications not tested.

Recommended Dilution:
Direct Immunofluorescence: 1:50 dilution range.
Dot Blot: 1:200
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2b	5i25	Highly Purified

Size		Storage	Shipping	Source	Host
50 Tests		4°C (-20°C Glycerol)	Blue Ice		Mouse

Concentration:

Not determined

Immunogen:

Mouse gp120, V3 loop specific

Purity:

Purified by gel exclusion chromatography.

Form

Supplied as a liquid in 10mM Tris, pH 8.0. No stabilizers or preservatives added. Labeled with FITC.

Specificity:

Recognizes native and recombinant HIV-1 gp120 (IIIB, MN, SF2, RF, CDC4) in Dot Blot assays and stains cell surface bound gp120 in HIV-1 infected SupT1 and primary HIV-1 infected human PBLs.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.