Technical Data
H6003-34G4
HIV-1 gp120 (Human Immunodeficiency Virus Type 1) (Gold)
Description:
Applications:
Suitable for use in Dot Blot, Immunohistochemistry and cell surface binding. Other applications have not been tested.

Recommended Dilutions:
Dot Blot: 10-100ng of gp120 in solid phase (membrane bound).
Immunohistochemistry: 1:50 to 1:100 dilution range of gold conjugate.
Optimal dilutions to be determined by the researcher.

Coupling Ratio:
OD520nm = 10; Protein content approx. 50ug

Storage and Stability:
May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Gold conjugates are light sensitive.
TypeIsotypeCloneGrade
MabIgG15i26Highly Purified
SizeStorageShippingSourceHost
1ml4C Do not freezeBlue IceMouse
Concentration:
Not Determined
Immunogen:
Purity:
95%
Form
Supplied as a liquid in 10mM Tris, pH 8.0, 5% BSA, 0.05% PEG, 0.02% sodium azide. Labeled with Colloidal Gold (approx. 30nm mean diameter).
Specificity:
Binds to V3-loop of HIV-1 gp120/gp160. Crossreactivity: MN, IIIB, RF, SF2 and other primary HIV-1 isolates. Cell surface binding was performed with HIV-1 infected lymphocytes enriched from human PBL's.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.