Technical Data
H6003-35
HIV-1 gp120 (Human Immunodeficiency Virus Type 1)
Description:
Human immunodeficiency virus type 1 (HIV-1) entry into target cells is directed by the envelope (Env) glycoproteins, which are present on the surface of HIV-1 virion or infected cells in the form of trimers consisting of gp120/gp41 complexes. The surface subunit, gp120, initiates the entry process by interacting sequentially with the CD4 receptor and a co-receptor CCR5 or CXCR4, thereby inducing a conformational change that allows the transmembrane (TM) gp41 subunit to mediate fusion between viral and target cell membranes. Cleavage of Env into its gp120 and gp41 components is necessary for activation of its fusogenic activity.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceGoat
Concentration:
~5mg/ml
Immunogen:
Highly purified HIV1 gp120.
Purity:
Purified by ion exchange chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Recognizes gp120. No reactivty to human T or B cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Jenkinson, S. et al., (2003) J Biomol Screen 8:463-470.