Technical Data
H6003-39
HIV-1 gp120 (Human Immunodeficiency Virus-1)
Description:
Human immunodeficiency virus type 1 (HIV-1) entry into target cells is directed by the envelope (Env) glycoproteins, which are present on the surface of HIV-1 virion or infected cells in the form of trimers consisting of gp120/gp41 complexes. The surface subunit, gp120, initiates the entry process by interacting sequentially with the CD4 receptor and a co-receptor CCR5 or CXCR4, thereby inducing a conformational change that allows the transmembrane (TM) gp41 subunit to mediate fusion between viral and target cell membranes. Cleavage of Env into its gp120 and gp41 components is necessary for activation of its fusogenic activity.

Applications:
Suitable for use in ELISA (capture) and Western Blot. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

End-Point Titer: >1:1000 by Western Blot

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
1ml-20CBlue IceGoat
Concentration:
~4-5mg/ml
Immunogen:
Purified native gp120 from strain IIIB.
Purity:
Purified IgG fraction to a purity of > 95%.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins have been added.
Specificity:
Recognizes HIV-1 gp120 (predominate gp120 band in WB). Does not cross-react with human T or B cells, human or bovine sera.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.