Technical Data
H6098-06A
HLA Class 1 Antigen A1,A36 (Biotin)
Description:
The human leukocyte antigen system (HLA) is the name of the major histocompatibility complex (MHC) in humans. The superlocus contains a large number of genes related to immune system function in humans. This group of genes resides on chromosome 6, and encodes cell-surface antigen-presenting proteins and many other genes. The HLA genes are the human versions of the MHC genes that are found in most vertebrates (and thus are the most studied of the MHC genes). The proteins encoded by certain genes are also known as antigens, as a result of their historic discovery as factors in organ transplantations. The major HLA antigens are essential elements for immune function. Different classes have different functions: HLA class I antigens (A, B & C) present peptides from inside the cell (including viral peptides if present). These peptides are produced from digested proteins that are broken down in the proteasomes. The peptides are generally small polymers, about 9 amino acids in length. Foreign antigens attract killer T-cells (also called CD8 positive- or cytotoxic T-cells) that destroy cells.

Applications:
Suitable for use in Flow Cytometry. Other potential applications include Cell Typing, Tissue Staining and Chimerism Studies. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Source:
Ascites fluid

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgM8.L.104Highly Purified
SizeStorageShippingSourceHost
250ul-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Purity:
Purified by Ion Exchange chromatography.
Form
Supplied as a liquid in PBS, 0.1% sodium azide, 1% BSA. Labeled with Biotin.
Specificity:
Recognizes human HLA Class 1 Antigen A1, A36.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: 1. Liu, G. et al., J. Immunotherapy (2003) 26;301-312. 2. Xu, Q. et al., (2009) Stem Cells 27:1734-1740. 3. Lui, G. et al., (2004) Cancer Res. 64; 4980-4986. 1. NCCLS Tentative Standard. "Leukocyte Differential Counting" Publication Number H20-T, NCCLS, Vol. 4, No. 11 (1984). 2. Pei, R., et al., Tissue Antigen 41: 200-203 (1993). 3. Pei, R., Woo, G. and Lee, J.H. "Detection of blood chimerism at a frequency of one per thousand by flow cytometry. Visuals of the clinical histocompatibility workshop", Paul I. Terasaki, Ed., pp.73-74 (1995). 4. Pei, R., et al., Tissue Antigens 50: 197-201 (1997).