Technical Data
H6098-07E
HLA Class 1 Antigen A2 (FITC)
Description:
Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Neat-1:10. Use 10ul of the suggested working dilution to label 10e6 cells or 100ul whole blood.
Optimal dilutions to be determined by the researcher.

Hybridoma:
NS1 myeloma cells fused with spleen cells from Balb/c mice.

Recommended Negative Controls:
I1904-84S: IgG2b, Mouse (FITC)

Source:
Tissue culture supernatant

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b3H2205Affinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~0.05mg/ml
Immunogen:
Papain solubilized HLA-A2. The epitope appears to include the carboxy-terminus of the alpha-2 helix and a turn on one of the underlying beta strands.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.1% sodium azide, before the addition of glycerol to 40%. Labeled with fluorescein isothiocyanate (FITC).
Specificity:
Recognizes the human HLA-A2 histocompatibility antigen.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Parham, P., Brodsky, F.M., Hum. Immunol. 3: 277-299 (1981). 2. Hogan, K.T., Brown, S.L., Hum Immunol. 33: 185-192 (1992). 3. Harig, S., et al., Blood 98: 2999-3005 (2001).