Technical Data
H6098-07F
HLA Class 1 Antigen A2 (PE)
Description:
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C.
The HLA A gene is part of the human HLA complex on chromosome 6 and there are a large number of variant alleles of this gene.

Applications:
Suitable for use in Flow Cytometry. Other applications have not been tested.

Recommended Dilution:
Flow Cytometry: Neat. Use 10ul to label 10e6 cells or 100ul whole blood.
Optimal dilutions to be determined by researcher.

Recommended Negative Control:
I1904-84H3: IgG2b Negative Control (PE)

Hybridoma:
NS1 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
Lyophilized powder may be stored at 4°C. Reconstitute by adding sterile ddH2O. Aliquot and store at 4°C. DO NOT FREEZE! Reconstituted product is stable for 12 months as an undiluted liquid. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b3H2205Affinity Purified
SizeStorageShippingSourceHost
100Tests4°C Do not freezeBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Papain solubilized HLA Class 1 Antigen A2 (HLA-A2)
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 1% BSA, 5% sucrose, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O.
Specificity:
Recognizes the human HLA-A2 histocompatibility antigen. The epitope recognized by this antibody has been studied extensively and would appear to include the carboxy-terminus of the alpha-2 helix and a turn on one of the underlying beta strands.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Parham, P. and Brodsky, F.M., Hum. Immunol. 3: 277-299 (1981). 2. Hogan, K.T. and Brown, S.L., Hum. Immunol. 33: 185-192 (1992). 3. Harig, S., et al., Blood 98: 2999-3005 (2001). 4. Pantenburg, B. et al. (2010) Am J Trop Med Hyg. 82:600­7. 5. Duncan, L.M. et al. (2010) J Immunol. 184: 6978­85. 6. Wang, B. et al. (2004) Blood. 104: 200­6. 7. Wooldridge, L. et al. (2007) Eur J Immunol. 37: 1323­33. 8. Wooldridge, L. et al. (2010) J Immunol. 184: 3357­66. 9. Popovic, J. et al. (2011) The only proposed T­cell epitope derived from the TEL­AML1 translocation is not naturally processed. Blood. May 25. [Epub ahead of print]. 10. Schmidt, J. et al. (2011) J Biol Chem. 286: 41723­35.