		Technical Data
H6098-10L	HLA Class 1 (SLA Class I) (FITC)

Description:
SLA-1 is expressed by all nucleated porcine cells, but not by erythrocytes.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: Neat; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Hybridoma:
SP2/0-Ag14 myeloma cells with spleen cells from Balb/c mice

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.

Type	Isotype	Clone	Grade
Mab	IgG1	10D416	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Porcine	Mouse

Concentration:

~0.1mg/ml

Immunogen:

Porcine peripheral blood mononuclear cells.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).

Specificity:

Recognizes a monomorphic epitope expressed by porcine MHC class I molecules (SLA-1). Recognizes human MHC Class I, including HLA-E.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Galiani, D. et al. (2002) A new monoclonal antibody (JM1E3) specific for porcine SLA Class I antigen recognises HLA Class I antigens and interferes with HLA recognition by human NK inhibitory receptors. In Leucocyte Typing VII. Edited by Mason. D. et al. Oxford University Press pp 437 - 439.