SLA-1 is expressed by all nucleated porcine cells, but not by erythrocytes.
Suitable for use in Flow Cytometry. Other applications not tested.
Flow Cytometry: Neat; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.
SP2/0-Ag14 myeloma cells with spleen cells from Balb/c mice
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
|Porcine peripheral blood mononuclear cells.|
|Purified by Protein G affinity chromatography.|
|Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).|
|Recognizes a monomorphic epitope expressed by porcine MHC class I molecules (SLA-1). Recognizes human MHC Class I, including HLA-E.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Galiani, D. et al. (2002) A new monoclonal antibody (JM1E3) specific for porcine SLA Class I antigen recognises HLA Class I antigens and interferes with HLA recognition by human NK inhibitory receptors. In Leucocyte Typing VII. Edited by Mason. D. et al. Oxford University Press pp 437 - 439.|