Technical Data
H6098-11
HLA Class 1 Antigen A2,A24
Description:
Applications:
This antibody has not been tested in methodologies other than Microcytotoxicity Test. Potential applications include Flow Cytometry, Cell Typing, Tissue Staining and Chimerism Studies.

Recommended Dilution:
For a cytotoxic working dilution, add 0.9ml of PBS, 1% BSA to 100ul of10X stock solution.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Note: FITC-labeled and purified versions of these antibodies are available on special order.
TypeIsotypeCloneGrade
MabIgM4i76Ascites
SizeStorageShippingSourceHost
100ul4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
Not determined
Immunogen:
Purity:
Ascites
Form
Supplied as a lyophilized powder from PBS, 1% BSA. Reconstitute with 100ul sterile ddH2O to make a 10X stock solution.
Specificity:
Recognizes human HLA Class 1 Antigen-A2,A24.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. NCCLS Tentative Standard. "Leukocyte Differential Counting" Publication Number H20-T, NCCLS, Vol. 4, No. 11 (1984). 2. Pei, R., et al., Amonospecific HLA-27 fluorescein isothiocyanate conjugated monoclonal antibody for rapid, simple and accurate HLA-B27 typing. Tissue Antigen 41: 200-203 (1993). 3. Pei, R., Woo, G. and Lee, J.H. "Detection of blood chimerism at a frequency of one per thousand by flow cytometry. Visuals of the clinical histocompatibility workshop", Paul I. Terasaki, Ed., pp.73-74 (1995). 4. Pei, R., Chen, T., Orpilla, J. and Lee, J.H. "A simultaneous negative and positive selection method that can detect chimerism at a frequency of 1 per 10,000 by flow cytometry", Tissue Antigens 50:197-201 (1997).