		Technical Data
H6098-14	HLA Class 1 Antigen A2,A3,A29(X-Reactive)

Description:
Applications:
Suitable for use in Flow Cytometry, Cell Typing, Tissue Staining, and Chimerism Studies. Other applications have not been tested.

Recommended Dilutions:
Cytotoxicity: Add 0.9ml of 1% BSA in PBS to 100ul of10X working dilution.
Optimal dilutions to be determined by the researcher.

Cytotoxicity:
Expected Results:
Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction.
Limitations:
Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.

Source:
Ascites fluid

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgM	4i79	Ascites

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Mouse

Concentration:

Not Determined

Immunogen:

HLA Class 1 Antigen-A2, A3, A29.

Purity:

Ascites

Form

Supplied as a lyophilized powder from PBS, 1% BSA. Reconstitute with 100ul sterile ddH2O to make a 10X stock solution.

Specificity:

Recognizes human HLA Class 1 Antigen-A2,A3,A29. Additional crossreactivity occurs with related proteins.. Specificity was determined at a 1:10 dilution by the microcytotoxicity test under standard NIH conditions.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. NCCLS Tentative Standard. "Leukocyte Differential Counting" Publication Number H20-T, NCCLS, Vol. 4, No. 11 (1984). 2. Pei, R., et al., Amonospecific HLA-27 fluorescein isothiocyanate conjugated monoclonal antibody for rapid, simple and accurate HLA-B27 typing. Tissue Antigen 41: 200-203 (1993). 3. Pei, R., Woo, G. and Lee, J.H. "Detection of blood chimerism at a frequency of one per thousand by flow cytometry. Visuals of the clinical histocompatibility workshop", Paul I. Terasaki, Ed., pp.73-74 (1995). 4. Pei, R., Chen, T., Orpilla, J. and Lee, J.H. "A simultaneous negative and positive selection method that can detect chimerism at a frequency of 1 per 10,000 by flow cytometry", Tissue Antigens 50:197-201 (1997).