Technical Data
H6098-15
HLA Class 1 Antigen A2,A69 (IgG)
Description:
Applications:
Suitable for use in Cell Typing, Tissue Staining, and Chimerism Studies.

Recommended Dilution:
For a cytotoxic working dilution, add 0.9ml of 1% BSA in PBS to 100ul of10X working dilution. Store aliquots at -20°C.

Expected Results:
Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction.

Limitations:
Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a4i80Ascites
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanMouse
Concentration:
Not Determined
Immunogen:
HLA Class 1 Antigen-A2, A69.
Purity:
Ascites
Form
Supplied as a lyophilized powder in PBS, 1% BSA. Reconstitute to 100ul sterile dH2O to make a 10X stock solution.
Specificity:
Recognizes human HLA Class 1 Antigen-A2,A69.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. NCCLS Tentative Standard. "Leukocyte Differential Counting" Publication Number H20-T, NCCLS, Vol. 4, No. 11 (1984). 2. Pei, R., et al., Amonospecific HLA-27 fluorescein isothiocyanate conjugated monoclonal antibody for rapid, simple and accurate HLA-B27 typing. Tissue Antigen 41: 200-203 (1993). 3. Pei, R., Woo, G. and Lee, J.H. "Detection of blood chimerism at a frequency of one per thousand by flow cytometry. Visuals of the clinical histocompatibility workshop", Paul I. Terasaki, Ed., pp.73-74 (1995). 4. Pei, R., Chen, T., Orpilla, J. and Lee, J.H. "A simultaneous negative and positive selection method that can detect chimerism at a frequency of 1 per 10,000 by flow cytometry", Tissue Antigens 50:197-201 (1997).