Technical Data
H6098-20
HLA Class 1 Antigen A3 (R0.90)
Description:
The human leukocyte antigen system (HLA) is the name of the major histocompatibility complex (MHC) in humans and is an essential element for immune function. This group of genes re- sides on chromosome 6, and encodes cell-surface antigen- presenting proteins and many other genes. Different classes have different functions: HLA class I antigens (A, B & C) present peptides from inside the cell, including viral peptides if present. These peptides are produced from digested proteins that are broken down in the proteasomes. Foreign antigens attract killer T-cells (also called CD8+ or cytotoxic T-cells) that destroy cells.

Applications:
This antibody has not been tested in methodologies other than Microcytotoxicity Test. Potential applications include Flow Cytometry, Cell Typing, Tissue Staining and Chimerism Studies.

Recommended Dilutions:
Cytotoxic Working Dilution: Add 0.9ml 1% BSA in PBS to 100ul of10X stock solution.
Optimal dilutions to be determined by the researcher.

Cytotoxicity:
Expected Results:
Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction.
Limitations:
Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgM4i85Ascites
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
Not Determined
Immunogen:
Purity:
Ascites
Form
Supplied as a lyophilized powder. Reconstitute with 100ul ddH2O to make a 10X stock solution.
Specificity:
Recognizes human HLA Class 1 Antigen A3. Specificity was determined at a 1:10 dilution by the microcytotoxicity test under standard NIH conditions.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. NCCLS Tentative Standard. "Leukocyte Differential Counting" Publication Number H20-T, NCCLS, Vol. 4, No. 11, 1984. 2. Pei, R., et al., Tissue Antigen, 41: 200-203, 1993. 3. Pei, R., et al., "Detection of blood chimerism at a frequency of one per thousand by flow cytometry. Visuals of the clinical histocompatibility workshop", Paul I. Terasaki, Ed., pp.73-74, 1995. 4. Pei, R., et al., Tissue Antigens 50: 197-201, 1997.