Technical Data
H6098-20A
HLA Class 1 Antigen A3 (R0.90) (Biotin)
Description:
The human leukocyte antigen system (HLA) is the name of the major histocompatibility complex (MHC) in humans and is an essential element for immune function. This group of genes re- sides on chromosome 6, and encodes cell-surface antigen- presenting proteins and many other genes. Different classes have different functions: HLA class I antigens (A, B & C) present peptides from inside the cell, including viral peptides if present. These peptides are produced from digested proteins that are broken down in the proteasomes. Foreign antigens attract killer T-cells (also called CD8+ or cytotoxic T-cells) that destroy cells.

Applications:
Suitable for use in Flow Cytometry. Other potential applications include Cell Typing, Tissue Staining and Chimerism Studies. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Source:
Ascites fluid

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgM4i153Highly Purified
SizeStorageShippingSourceHost
250ul-20CBlue IceHumanMouse
Concentration:
Not Determined
Immunogen:
Purity:
Purified by Ion Exchange chromatography.
Form
Supplied as a liquid in PBS, 0.5% BSA, 0.05% sodium azide. Labeled with Biotin.
Specificity:
Recognizes HLA Class 1 Antigen-A3. R-Value: 0.9
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. NCCLS Tentative Standard. "Leukocyte Differential Counting" Publication Number H20-T, NCCLS, Vol. 4, No. 11 (1984). 2. Pei, R., et al., Amonospecific HLA-27 fluorescein isothiocyanate conjugated monoclonal antibody for rapid, simple and accurate HLA-B27 typing. Tissue Antigen 41: 200-203 (1993). 3. Pei, R., Woo, G. and Lee, J.H. "Detection of blood chimerism at a frequency of one per thousand by flow cytometry. Visuals of the clinical histocompatibility workshop", Paul I. Terasaki, Ed., pp.73-74 (1995). 4. Pei, R., Chen, T., Orpilla, J. and Lee, J.H. "A simultaneous negative and positive selection method that can detect chimerism at a frequency of 1 per 10,000 by flow cytometry", Tissue Antigens 50:197-201 (1997).