Description:
Applications:
Suitable for use in Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG1 | 8L252 | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 100ug | | -20°C | Blue Ice | | Mouse |
|
| Concentration: |
| As reported |
| Immunogen: |
| PHA-activated peripheral blood lymphocytes. |
| Purity: |
| Purified by immunoaffinity chromatography. |
| Form |
| 15mM Sodium Azide; PBS. pH 7.4 |
| Specificity: |
| Reacts with all human classical MHC Class I molecules in native cell-surface forms. Positive in immunoblotting with many (not all) MHC Class I allelic forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blot). Positive in Western blot (non-reducing conditions) with most HLA-B and HLA-C molecules, but not HLA-A. Epitope specificity is very similar to classical antibody W6/32. Species Crossreactivity: Crossreacts with Human. Not yet tested in other species. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
This product has been used in:Tran TM et al. The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex. Immunogenetics 53:440-6 (2001).
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