Suitable for use in Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
| As reported|
|PHA-activated peripheral blood lymphocytes.|
|Purified by immunoaffinity chromatography.|
|15mM Sodium Azide; PBS. pH 7.4|
|Reacts with all human classical MHC Class I molecules in native cell-surface forms. Positive in immunoblotting with many (not all) MHC Class I allelic forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blot). Positive in Western blot (non-reducing conditions) with most HLA-B and HLA-C molecules, but not HLA-A. Epitope specificity is very similar to classical antibody W6/32. Species Crossreactivity: Crossreacts with Human. Not yet tested in other species.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
This product has been used in:Tran TM et al. The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex. Immunogenetics 53:440-6 (2001). <11685454>|