		Technical Data
H6098-72L	HLA-G

Description:
Applications:
Suitable for use in Immunohistochemistry and Western Blot. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry (Paraffin sections): 1:60-1:100. Incubate 1 hour at RT. Stain by standard ABC technique. Requires heat antigen retrieval in 0.01 citrate buffer (microwave 4x2 minutes).
Optimal dilution to be determined by the researcher.

IHC Positive Control:
Human placenta-extravillous cytotrophoblast.

Storage and Stability:

May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	6D463	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		4°C (-20°C Glycerol)	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Human recombinant HLA-G denatured heavy chain

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.

Specificity:

Recognizes human denatured HLA-G heavy chain.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules: S. Fournel, et al.; Tissue Antigens 55, 510 (2000) 2. Melanomas and melanoma cell lines do not express HLA-G, and the expression cannot be induced by gammaIFN treatment: G. Frumento, et al.; Tissue Antigens 56, 30 (2000) 3. Disulfide bond-mediated dimerization of HLA-G on the cell surface: J.E. Boyson, et al.; PNAS 99, 16180 (2002)