Technical Data
H6098-72L
HLA-G
Description:
Applications:
Suitable for use in Immunohistochemistry and Western Blot. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry (Paraffin sections): 1:60-1:100. Incubate 1 hour at RT. Stain by standard ABC technique. Requires heat antigen retrieval in 0.01 citrate buffer (microwave 4x2 minutes).
Optimal dilution to be determined by the researcher.

IHC Positive Control:
Human placenta-extravillous cytotrophoblast.

Storage and Stability:

May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

TypeIsotypeCloneGrade
MabIgG16D463Affinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human recombinant HLA-G denatured heavy chain
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Specificity:
Recognizes human denatured HLA-G heavy chain.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Comparative reactivity of different HLA-G monoclonal antibodies to soluble HLA-G molecules: S. Fournel, et al.; Tissue Antigens 55, 510 (2000) 2. Melanomas and melanoma cell lines do not express HLA-G, and the expression cannot be induced by gammaIFN treatment: G. Frumento, et al.; Tissue Antigens 56, 30 (2000) 3. Disulfide bond-mediated dimerization of HLA-G on the cell surface: J.E. Boyson, et al.; PNAS 99, 16180 (2002)