Technical Data
H6100-27
HLA Class 2 Antigen DR4 (X-Reactive)
Description:
Applications:
This antibody has not been tested in methodologies other than Microcytotoxicity Test. Potential applications include Flow Cytometry, Cell Typing, Tissue Staining and Chimerism Studies.
Other applications not tested.

Recommended Dilutions:
Cytotoxicity: Add 0.9ml of 1% BSA in PBS to 100ul of 10X working dilution.
Optimal dilutions to be determined by the researcher.

Cytotoxicity:
Expected Results:
Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction.
Limitations:
Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.

Source:
Ascites fluid

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgM8.L.335Ascites
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Purity:
Ascites
Form
Supplied as a lyophilized powder from PBS, 1% BSA. Reconstitute with 100ul sterile ddH2O to make a 10X stock solution.
Specificity:
Recognizes HLA Class 2 Antigen-DR4. Additional crossreactivity occurs with related proteins, such as +0401, +0403,+/- DR1, -/+0402, -/+ DR7. Specificity was determined at a 1:10 dilution by the microcytotoxicity test under standard NIH conditions.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. NCCLS Tentative Standard. "Leukocyte Differential Counting" Publication Number H20-T, NCCLS, Vol. 4, No. 11 (1984). 2. Pei, R., et al., Amonospecific HLA-27 fluorescein isothiocyanate conjugated monoclonal antibody for rapid, simple and accurate HLA-B27 typing. Tissue Antigen 41: 200-203 (1993). 3. Pei, R., Woo, G. and Lee, J.H. "Detection of blood chimerism at a frequency of one per thousand by flow cytometry. Visuals of the clinical histocompatibility workshop", Paul I. Terasaki, Ed., pp.73-74 (1995). 4. Pei, R., Chen, T., Orpilla, J. and Lee, J.H., "A simultaneous negative and positive selection method that can detect chimerism at a frequency of 1 per 10,000 by flow cytometry", Tissue Antigens 50: 197-201 (1997).